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Acquisition of epibiotic bacteria along the life cycle of the hydrothermal shrimp Rimicaris exoculata ArchiMer
Guri, Mathieu; Durand, Lucile; Cueff-gauchard, Valerie; Zbinden, Magali; Crassous, Philippe; Shillito, Bruce; Cambon-bonavita, Marie-anne.
The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent sites, we analyzed the epibionts diversity at different moult stages and also in the first stages of the shrimp life (eggs, hatched eggs (with larvae) and juveniles). Hatched eggs associated with young larvae were collected for the first time directly from gravid females at the Logachev vent site during the Serpentine cruise. An approach using 16S rRNA clone libraries, scanning and transmission electron...
Tipo: Text Palavras-chave: Symbiosis; Larvae; Methanotrophic symbiont; Rimicaris exoculata; Transmission pathways.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00072/18318/15997.pdf
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Chemical and thermal description of the environment of the Genesis hydrothermal vent community (13 degrees N, EPR) ArchiMer
Sarradin, Pierre-marie; Caprais, Jean-claude; Briand, Patrick; Gaill, Françoise; Shillito, Bruce; Desbruyeres, Daniel.
The objective of this study is to describe the chemical and physical environment surrounding the vent organisms at the Genesis site (EPR, 2640 m). The main chimney is colonized by Riftia pachyptila, fishes Zoarcidae and crabs Bythograeidae. The top of the smoker is covered with tubes of polychaetes Alvinellidae, the frontier zone by limpet gastropods. Temperature measurements and water sampling were made on an axis along the chimney. The environment was characterized using relationships between chemical concentrations and temperature to provide a chemo-thermal model of the site. Discrete temperature ranges were 1-1.6 degrees C in sea water, 1.6-10 degrees C among Riftia plumes (up to 25 degrees C at the tube base), 7-91 degrees C close to the alvinellid...
Tipo: Text Palavras-chave: Hydrothermal ecosystem; Hydrogen sulphide; Carbon dioxide; Temperature.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00186/29764/28200.pdf
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Estimating Symbiont Abundances and Gill Surface Areas in Specimens of the Hydrothermal Vent Mussel Bathymodiolus puteoserpentis Maintained in Pressure Vessels ArchiMer
Duperron, Sebastien; Quiles, Adrien; Szafranski, Kamil M.; Leger, Nelly; Shillito, Bruce.
The hydrothermal vent mussel Bathymodiolus puteoserpentis hosts gill-associated sulfur- and methane-oxidizing bacteria which sustain host nutrition and allow it to reach high densities at various sites along the northern Mid-Atlantic Ridge. Previous studies have demonstrated that in similar dual symbioses, relative abundances of each bacterial type could change following variations in symbiont substrate availabilities. In this study, pressurized recovery and incubations in pressure vessels were used to test whether B. puteoserpentis symbionts displayed similar behavior in the presence of symbiont substrates. The relative abundances of both types of symbionts were analyzed using fluorescence in situ hybridization (FISH) and group-specific gene copy numbers...
Tipo: Text Palavras-chave: Symbiosis; In vivo experiments; Pressurized recovery; Bivalve; Mid-Atlantic Ridge.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00484/59533/62525.pdf
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EXtreme ecosystem studies in the deep OCEan: Technological Developments. EXOCET/D ArchiMer
Sarradin, Pierre-marie; Sarrazin, Jozee; Cadiou, Jean-francois; Olu, Karine; Desbruyeres, Damien; Rigaud, Vincent; Drogou, Jean-francois; Lecornu, Fabrice; Rolin, Jean-francois; Vuillemin, Renaud; Cambon-bonavita, Marie-anne; Sauter, E; Von Juterzenka, K; Boetius, A; Santos, Rs; Colaco, Ana; Pascoal, A; Oliveira, Paulo; Shillito, Bruce; Zal, F; Schultz, A; Taylor, P; Lane, D; Loke, Rob; Du Buf, H; Waldmann, C; Cormack, A; Sanfilippo, L; Masson, M.
The general objective of EXOCET/D is to develop, implement and test specific instruments aimed at exploring, describing, quantifying and monitoring biodiversity in deep-sea fragmented habitats as well as at identifying links between community structure and environmental dynamics. Onboard experimental devices will complement the approach, enabling experiments on species physiology. The EXOCET/D working fields include: video and acoustic imagery, in situ analysis of physico-chemical factors, quantitative sampling of macroand micro-organisms, in vivo experiments, integration of multidisciplinary data, implementation on European deep-submersibles and a final phase of technical and scientific validation
Tipo: Text Palavras-chave: Deep sea; Ecosystem; Imagery; In situ analysis; Faunal sampling; Submersibles; Observatory.
Ano: 2004 URL: https://archimer.ifremer.fr/doc/2007/publication-3600.pdf
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Heat-shock response and temperature resistance in the deep-sea vent shrimp Rimicaris exoculata ArchiMer
Ravaux, Juliette; Gaill, Françoise; Le Bris, Nadine; Sarradin, Pierre-marie; Jollivet, Didier; Shillito, Bruce.
The shrimp Rimicaris exoculata swarms around hydrothermal black smoker chimneys at most vent sites along the Mid-Atlantic Ridge. This species maintains close proximity to the hydrothermal fluid, where temperatures can reach 350degreesC and steep thermal and chemical gradients are expected. We performed in vivo experiments in pressurized aquaria to determine the upper thermal limit [critical thermal maximum (CTmax)] of R. exoculata and to investigate some characteristics of the shrimp stress response to heat exposure. These experiments showed that the shrimp does not tolerate sustained exposure to temperatures in the 33-37degreesC range (CTmax). A heat-inducible stress protein belonging to the hsp70 family was identified in R. exoculata, and its synthesis...
Tipo: Text Palavras-chave: Heat shock; Shrimp; Rimicaris exoculata; Chaperone; IPOCAMP; Caridae; Crustacea; Thermal stress; Hydrothermal vent.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-417.pdf
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High rates of apoptosis visualized in the symbiont-bearing gills of deep-sea Bathymodiolus mussels ArchiMer
Piquet, Berenice; Shillito, Bruce; Lallier, Francois H.; Duperron, Sebastien; Andersen, Ann C..
Symbiosis between Bathymodiolus and Gammaproteobacteria allows these deep-sea mussels to live in toxic environments such as hydrothermal vents and cold seeps. The quantity of endosymbionts within the gill-bacteriocytes appears to vary according to the hosts environment; however, the mechanisms of endosymbiont population size regulation remain obscure. We investigated the possibility of a control of endosymbiont density by apoptosis, a programmed cell death, in three mussel species. Fluorometric TUNEL and active Caspase-3-targeting antibodies were used to visualize and quantify apoptotic cells in mussel gills. To control for potential artefacts due to depressurization upon specimen recovery from the deep-sea, the apoptotic rates between mussels recovered...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00484/59526/62500.pdf
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HOT 96 News ArchiMer
Gaill, Francoise; Felbeck, Horst; Desbruyeres, Daniel; Lallier, Francois; Toulmond, A.; Alayse, Anne-marie; Briand, Patrick; Brulport, Jean-pierre; Caprais, Jean-claude; Chevaldonne, P.; Coail, Jean-yves; Cosson, R.; Crassous, Philippe; Delachambre, J.; Durif, C.; Echardour, L.; Herve, G.; Hourdez, Stephane; Jollivet, D.; Kerdoncuff, Jacques; Khripounoff, Alexis; Lechaire, J.p.; Pruski, Audrey; Ravaux, Juliette; Sarradin, Pierre-marie; Shillito, Bruce; Toullec, J.y.; Arndt, C.; Fisher, C.; Lutz, Richard; Childress, J..
Tipo: Text
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00179/29003/27431.pdf
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Is It First the Egg or the Shrimp? – Diversity and Variation in Microbial Communities Colonizing Broods of the Vent Shrimp Rimicaris exoculata During Embryonic Development ArchiMer
Methou, Pierre; Hernández-ávila, Ivan; Aube, Johanne; Cueff-gauchard, Valerie; Gayet, Nicolas; Amand, Louis; Shillito, Bruce; Pradillon, Florence; Cambon-bonavita, Marie-anne.
Rimicaris exoculata is one of the most well-known and emblematic species of endemic vent fauna. Like many other species from these ecosystems, Rimicaris shrimps host important communities of chemosynthetic bacteria living in symbiosis with their host inside the cephalothorax and gut. For many of these symbiotic partners, the mode of transmission remains to be elucidated and the starting point of the symbiotic relationship is not yet defined, but could begin with the egg. In this study, we explored the proliferation of microbial communities on R. exoculata broods through embryonic development using a combination of NGS sequencing and microscopy approaches. Variations in abundance and diversity of egg microbial communities were analyzed in broods at...
Tipo: Text Palavras-chave: Hydrothermal; Shrimp; Microbial colonization; Alvinocarididae; Egg development.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00489/60067/63352.pdf
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Long-term maintenance and public exhibition of deep-sea Hydrothermal fauna: the AbyssBox project ArchiMer
Shillito, Bruce; Ravaux, Juliette; Sarrazin, Jozee; Zbinden, Magali; Sarradin, Pierre-marie; Barthelemy, Dominique.
The AbyssBox project aims to provide the first permanent public exhibition of live deep-sea hydrothermal fauna maintained at in situ pressure. AbyssBox is a pressurized aquarium designed to function permanently. Here we present details of the project after the public exhibition functioned for more than three years at Océanopolis aquarium in Brest, France. We also describe the AbyssBox pressure aquarium, and provide data and observations on vent shrimp (Mirocaris fortunata) and crabs (Segonzacia mesatlantica) that were sampled from 1700 m depth at the Lucky Strike vent field (Mid-Atlantic Ridge) during different cruises. While mortalities exceeded 50% during the first days following sampling, the remaining animals appeared to acclimate fairly well. Some...
Tipo: Text Palavras-chave: AbyssBox; Aquaria; Aquariology; Deep waters; Hydrostatic pressure; Hydrothermal vents; Public exhibition; Scientific experiments.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00268/37967/36046.pdf
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Microbial diversity associated with the hydrothermal shrimp Rimicaris exoculata gut and occurrence of a resident microbial community ArchiMer
Durand, Lucile; Zbinden, Magali; Cueff-gauchard, Valerie; Duperron, Sebastien; Roussel, Erwan; Shillito, Bruce; Cambon-bonavita, Marie-anne.
Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full of sulphides and iron-oxide particles and harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were performed on shrimps from Rainbow and Trans-Atlantic Geotraverse sites in order to expel the transient gut contents. Microbial communities associated with the gut of starved and reference shrimps were compared using 16S rRNA gene libraries and microscopic observations (light, transmission and scanning electron microscopy and FISH analyses). We show that the gut microbiota of shrimps from both sites included mainly Deferribacteres, Mollicutes,...
Tipo: Text Palavras-chave: Deferribacteres; Midgut epibiosis; Mollicutes; Proteobacteria; Rimicaris exoculata; Starvation experiment.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00000/11142/7919.pdf
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Molecular identification of differentially regulated genes in the hydrothermal-vent species Bathymodiolus thermophilus and Paralvinella pandorae in response to temperature ArchiMer
Boutet, Isabelle; Jollivet, Didier; Shillito, Bruce; Moraga, Dario; Tanguy, Arnaud.
Background: Hydrothermal vents and cold seeps represent oases of life in the deep-sea environment, but are also characterized by challenging physical and chemical conditions. The effect of temperature fluctuations on vent organisms in their habitat has not been well explored, in particular at a molecular level, most gene expression studies being conducted on coastal marine species. In order to better understand the response of hydrothermal organisms to different temperature regimes, differentially expressed genes (obtained by a subtractive suppression hybridization approach) were identified in the mussel Bathymodiolus thermophilus and the annelid Paralvinella pandorae irlandei to characterize the physiological processes involved when animals are subjected...
Tipo: Text Palavras-chave: Oyster crassostrea gigas; Heat shock protein; Mid atlantic ridge; Cytosolic malate dehydrogenases; Extracellular hemoglobins; Lipid peroxidation; Northeast pacific; Enzyme activities; Arginine kinase; Dual symbiosis.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00129/24014/21970.pdf
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Temperature resistance of Hesiolyra bergi, a polychaetous annelid living on deep-sea vent smoker walls ArchiMer
Shillito, Bruce; Jollivet, Didier; Sarradin, Pierre-marie; Rodier, Philippe; Lallier, François; Desbruyeres, Daniel; Gaill, Françoise.
For the first time, in vivo heat-exposure experiments were conducted on the hydrothermal vent polychaete Hesiolyra bergi from the hottest part of the vent biotope. Using a pressurised incubator equipped with video-facilities, we found that H. bergi, which forages around and in the tubes of the thermophilic Alvinella sp., became hyperactive once temperature exceeded 35 degreesC and further lost co-ordination in the 41 to 46 degreesC interval, just before death occurred. Another exposure experiment at 39 degreesC for 3 to 4 h led to 80 % mortality (max) 9 h after heat shock, and 100 % thereafter. In view of the much higher temperatures recorded in this organism's habitat, these results suggest that tolerance to high temperatures (exceeding 40 degreesC) is...
Tipo: Text Palavras-chave: IPOCAMP; Adaptation to heat; Behaviour; Hydrothermal vents.
Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-811.pdf
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